Journal: International Journal of Nanomedicine

Article Title: Hollow Cu 2-x Se/PDA/IR820 Composite Nanoparticles with pH Response for Multimodal Synergistic Treatment of Esophageal Cancer

doi: 10.2147/IJN.S576500

Figure Lengend Snippet: In vitro antitumor effects of Cu 2-x Se/PDA/IR820 on KYSE150 cells. ( A ) Cell viability of KYSE150 and HUVECs after 6 h incubation with Cu 2-x Se/PDA/IR820, showing selective cytotoxicity toward cancer cells. ( B ) KYSE150 cell viability following 6 h treatment with Cu 2-x Se/PDA/IR820 and subsequent NIR irradiation, fitted with a nonlinear dose–response curve using a logistic model. ( C ) Confocal images of KYSE150 cells incubated with Cu 2-x Se/PDA/DiD (15 μg/mL) for 0, 2, 4, 6 and 8h. Nuclei are stained with DAPI (blue), lysosomes with Lyso-Tracker Green (green), and arrows indicate colocalization of nanoparticles within lysosomes. Scale bar = 20 μm. ( D ) EdU incorporation assay showing proliferating cells (green) and nuclei (blue) under different treatment conditions. Scale bar = 20 μm. ( E ) Confocal images of F-actin morphology in KYSE150 cells after various treatments; F-actin labeled with FITC-ghost pen cyclic peptide (green) and nuclei with DAPI (blue). Scale bar = 20 μm. ( F ) Live/dead staining of KYSE150 cells; live cells in green, dead cells in red. Scale bar = 100 μm. ( G ) Flow cytometry analysis of apoptosis in KYSE150 cells stained with Annexin V-FITC/PI, distinguishing early and late apoptotic populations. Data are presented as mean ± SD (n = 3).

Article Snippet: Human umbilical vein endothelial cells (HUVECs) and the murine esophageal carcinoma cell line AKR were purchased from ATCC (Manassas, VA, USA).

Techniques: In Vitro, Incubation, Irradiation, Staining, Labeling, Flow Cytometry